Creative Biolabs, a biotech company specialized in providing custom biotechnology and pharmaceutical services that cover the full scope of biotechnology needs, announces a novel strategy of manufacturing membrane protein antibodies with MagicTM Nanodiscs-based membrane protein purification service. With years of experience in membrane protein antibody production, Creative Biolabs has successfully developed MagicTM Nanodiscs-based membrane protein purification service, a comparatively new approach to manufacture membrane protein antibodies. MagicTM Nanodiscs are phospholipid bilayer membrane structures composed of membrane scaffold proteins (MSPs) and phospholipid molecules. Through this special structure, membrane protein can be integrated into MagicTM Nanodiscs, maintaining their biological activity and providing a powerful technical support for membrane protein research. This has overcome the biggest bottlenecks in membrane protein research. MagicTM Nanodiscs have sufficient and unique advantages compared with other membrane protein purification approaches. They can provide an excellent and nearly natural environment to keep membrane proteins stable. Because of their unique features, MagicTM Nanodiscs have broad application prospects. They can be applied to custom membrane protein antibody, analyze antagonists and conformational dynamics, characterize crystallization, as well as study ligand binding and protein interaction or activation. What’s more, MagicTM Nanodiscs can also be used to reconstitute membrane proteins such as GPCRs. Briefly, the MagicTM Nanodisc-solubilized proteins can be directly purified without using detergents. The completed assembly allows for the access to the physiologically intracellular and extracellular faces of the protein, which is its unique characteristic suit to G proteins and other types of protein with their own interaction partners. “We now have two methods to reconstitute membrane proteins into MagicTM Nanodiscs”, said Dr. Monika Müller, scientific officer of Creative Biolabs, “assembling the membrane proteins solubilized in the detergent and combing MagicTM Nanodiscs with cell-free expression systems”. Assemble the membrane proteins solubilized in the detergent Solubilize and purify the membrane proteins with a suitable detergent, following which membrane scaffo ld proteins and phospholipids are added into the mixture. After that, the MagicTM Nanodiscs containing the membrane proteins will assemble automatically. Combe MagicTM Nanodiscs with cell-free expression systems Express the membrane proteins in a cell-free system with the addition of pre-assembled MagicTM Nanodiscs that contains the integrated membrane proteins. In the method, the detergent is not needed. Moreover, the cell-free expression systems provide the possibility to introduce modifications into membrane proteins, such as biotinylation or isotope labelling. About Creative Biolabs Creative Biolabs is a professional service provider in developing highly specific, high affinity antibody from all popular antibody production species, including rabbit, chicken, llama, camel, alpaca, cow, dog, mouse, rat, sheep, monkey, human, and even shark. Creative Biolabs also has years of experience in developing antibody proteins. |
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November 2016
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